Generator

Part:BBa_K861038

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-08)

IPTG induced S-FadA and S-FadB

To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combinations with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. This part was used to produce protein FadA and FadB protein in Salmonella enterica LT2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 168
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2391
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1621


[edit]
Categories
Parameters
n/aIPTG induced S-FadA and S-FadB